Journal article
The antiepileptic medications carbamazepine and phenytoin inhibit native sodium currents in murine osteoblasts
SJ Petty, CJ Milligan, M Todaro, KL Richards, PK Kularathna, CN Pagel, CR French, EL Hill-Yardin, TJ O'Brien, JD Wark, EJ Mackie, S Petrou
Epilepsia | WILEY | Published : 2016
DOI: 10.1111/epi.13474
Abstract
Objective: Fracture risk is a serious comorbidity in epilepsy and may relate to the use of antiepileptic drugs (AEDs). Many AEDs inhibit ion channel function, and the expression of these channels in osteoblasts raises the question of whether altered bone signaling increases bone fragility. We aimed to confirm the expression of voltage-gated sodium (NaV) channels in mouse osteoblasts, and to investigate the action of carbamazepine and phenytoin on NaV channels. Methods: Immunocytochemistry was performed on primary calvarial osteoblasts extracted from neonatal C57BL/6J mice and additional RNA sequencing (RNASeq) was included to confirm expression of NaV. Whole-cell patch-clamp recordings were ..
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Grants
Awarded by NHMRC Centre for Research Excellence Grant
Funding Acknowledgements
The authors would like to sincerely acknowledge the following organizations for funding received during the development of this study including: the Molly McDonnell Foundation Scholarship (2009), DW Keir Fellowship in Medical Research at The Royal Melbourne Hospital (January 2010-June 2012), University of Melbourne Early Career Researcher (ECR) grant (2010), Brain Foundation Research Grant-in-Aid (2011), The Royal Melbourne Hospital Home Lottery Grants-in-Aid (2012), RACP Servier Barry Young Fellowship in Neuroscience (2012), The Royal Melbourne Hospital Victor Hurley Grants-in-Aid (2013), MBC Postdoctoral Medical Research Fellowship at The Melbourne Brain Centre RMH (June 2012-2014), and (NHMRC Centre for Research Excellence Grant 1001216). Dr. Sandra Petty held the Thwaites Gutch Fellowship at Ormond College Parkville 2011-2015.